Plasmid Linkage of Proteinase and Lactose Fermentation in Streptococcus lactis NCDO 1404
نویسندگان
چکیده
منابع مشابه
Plasmids in Streptococcus lactis: evidence that lactose metabolism and proteinase activity are plasmid linked.
Populations of lactose positive (Lac+) and proteinase positive (Prt+) cells from Streptococcus lactis M18, C10, and ML3 grown at 39 degrees C gave rise to increasing proportions of Lac- Prt- clones. The deficiencies did not appear until after a number of generations at the elevated temperature, and the rate depended on the strain.Lac- Prt+ and Lac+ Prt- mutants were isolated after treatment wit...
متن کاملTransductional evidence for plasmid linkage of lactose metabolism in streptococcus lactis C2.
A lactose-negative (Lac-), proteinase-negative (Prt-) mutant, designated C145 was isolated from Streptococcus lactis C2 after treatment with nitrosoguanidine and ultraviolet irradiation. The mutant appeared to be cured of the prophage(s) present in S. lactis C2 based on non-inducibility by ultraviolet irradiation or mitomycin C. When cleared lysate material from C145 was subjected, to cesium ch...
متن کاملPlasmid distribution and evidence for a proteinase plasmid in Streptococcus lactis C2-1.
Five plasmids, distinguishable by their molecular weights (10(6), 2 x 10(6), 5 x 10(6), 10(7), 3 x 10(7), respectively) were isolated from Streptococcus lactis C2. A spontaneous proteinase-negative derivative of this strain lacked the 10(7) plasmid.
متن کاملExtracellular Proteinase of Streptococcus Lactis.
Williamson, W. T. (North Carolina State of the University of North Carolina, Raleigh), S. B. Tove, and M. L. Speck. Extracellular proteinase of Streptococcus lactis. J. Bacteriol. 87:49-53. 1964.-Streptococcus lactis was shown to produce an extracellular proteolytic enzyme(s). A 120-fold purification of the proteinase was obtained from the cell-free culture medium by ammonium sulfate fractionat...
متن کاملSelection of Streptococcus lactis Mutants Defective in Malolactic Fermentation.
An enrichment medium and a new sensitive medium were developed to detect malolactic variants in different strains of lactic bacteria. Factors such as the concentration of glucose and l-malate, pH level, and the type of indicator dye used are discussed with regard to the kinetics of malic acid conversion to lactic acid. Use of these media allowed a rapid and easier screening of mutagenized strep...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1985
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(85)80860-2